Identification of mutations related to phytate accumulation in barley kernels

Aim. To conduct DNA marker testing on a sample of Ukrainian selection barley samples for identify various lpa-mutations that affect to the qualitative composition phosphorus in grains. Мethods. Isolation (CTAB method), polymerase chain reaction (PCR) and electrophoretic determination amplification products agarose gel identification lpa mutations Results. The conducted molecular genetic studies 20 breeding lines hulless made possible have lpa-1 lpa-2 mutations. presence such mutant genes determines low content organic (unavailable inorganic) an increased mineral (available) can be absorbed by human body. Conclusions. applied methods affecting phytate whole grain allow effective analysis used future crossing schemes.